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. 2009 Feb;149(2):642–652. doi: 10.1104/pp.108.130260

Figure 1.

Figure 1.

Isolation of protoplasts from Arabidopsis seedlings. A, Tissues from 14-d-old seedlings of Arabidopsis were collected and converted to protoplasts by a modified procedure of Chen and Halkier (2000). B, Protoplasts were purified by Suc density gradient centrifugation and collected at the interface of enzyme solution and W5 buffer (white arrows). C, Bright-field microscopy of protoplasts. Microphotographs were collected using a cooled CCD camera interfaced with the Zeiss Axioscope 2 plus microscope.