Figure 2.

The visualization of the efficiency of the transfection of protoplasts with the pSAT vector expressing nuclear-localized EGFP. Bright-field (A, Light) and fluorescent microphotographs (B, EGFP) were collected on the Zeiss Axioscope 2 plus microscope equipped with appropriate filter sets. White arrows indicate examples of nuclear-localized, EGFP-mediated fluorescence in the pSAT plasmid-transfected protoplasts. C, Superimposed bright-field and fluorescent images on A and B (Overlay) were created to demonstrate the transfection efficiency. D, Overlaid images of autofluorescence from chloroplasts and fluorescence from EGFP (EGFP-Chlorophyll) was created to demonstrate that green fluorescence derives solely from EGFP of the pSAT vector.