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. 2009 Feb;149(2):642–652. doi: 10.1104/pp.108.130260

Table I.

Comparison of the relative abundance of transcripts in protoplasts after RNAi using different DNA templates

Relative abundance of transcripts after RT-PCR was normalized to the level of ACT2, encoding actin, using GelQuant software.

DNA Templates for dsRNA Synthesis Size of dsRNA Depletion of Relative Transcript Abundance after RNAi
HXK1 HXK2
bp %
cDNA of HXK1 524 53 46
3′-UTR of HXK1 281 55 0
3′-UTR of HXK2 207 0 92
3′-UTR of HXK1 + 3′-UTR of HXK2 500 73 92