Table II.
List of oligos that were used to amplify DNA templates for in vitro dsRNA synthesis
| Size of dsRNA | Targeted Gene | Directions | Oligos (5′–3′) |
|---|---|---|---|
| bp | |||
| 547 | PCS1 | Forward | GCGCCTAATACGACTCACTATAGGGAGAGAACCTCTGGAAGTAGTGAAGGAA |
| Reverse | GCGCCTAATACGACTCACTATAGGGAGACTGTGAACTTACAAGACGAGGAAC | ||
| 540 | EGFP | Forward | GCGCCTAATACGACTCACTATAGGGAGAGCCAACACTTGTCACTACTTTCTC |
| Reverse | GCGCCTAATACGACTCACTATAGGGAGACATGTGGTCTCTCTTTTCGTTG | ||
| 548 | ECS1 | Forward | TAATACGACTCACTATAGGGAGATCATGCCAAAGGGGAGATAC |
| Reverse | TAATACGACTCACTATAGGGAGACTCTTCAACCGAACCTCTGG | ||
| 524 | HXK1 (cDNA) | Forward | AAGCTTTAATACGACTCACTATAGGGCTGCTTCCAAAATCAGGAGAAA |
| Reverse | GGATCCTAATACGACTCACTATAGGGGTAATGCTCAAACAATCCACCA | ||
| 281 | HXK1 (3′-UTR) | Forward | GCGCCTAATACGACTCACTATAGGGAGATCGCTATCAGAAAACGCCTAA |
| Reverse | GCGCCTAATACGACTCACTATAGGGAGACTCCAGTGAAGTGAGCTTTGA | ||
| 207 | HXK2 (3′-UTR) | Forward | GCGCCTAATACGACTCACTATAGGGAGATTGTTTTGCCGTTAGGGT |
| Reverse | GCGCCTAATACGACTCACTATAGGGAGAACAACATTGGCTGGTCGTTT | ||
| HXK1 (3′-UTR) + Linker | Forward | GCGCCTAATACGACTCACTATAGGGAGATCGCTATCAGAAAACGCCTAA | |
| Reverse | GGACCGTCACAATAGTACATAGAGAGGCCTCCAGTGAAGTGAGCTTTGA | ||
| HXK2 (3′-UTR) + Linker | Forward | GCCTCTCTATGTACTATTGTGACGGTCCTTGTTTTGCCGTTAGGGTTT | |
| Reverse | GCGCCTAATACGACTCACTATAGGGAGAACAACATTGGCTGGTCGTTT |