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NaPCCP is present in cytosolic and microsomal pollen tube fractions. Pollen tubes expressing both NaPCCP∷FLAG and cytosolic GFP were grown in culture, homogenized, and subjected to differential centrifugation. P10, 10,000g pellet; S10, 10,000g supernatant; S156, 156,000g supernatant; P156, 156,000g microsomal pellet. The anti-PCCP antibody (Ab) detects native NaPCCP (arrowhead) and NaPCCP∷FLAG (arrow). GFP was used as a cytosolic marker; vPPase was used as a microsome marker.
