Figure 5.

Hydrotropic response of gnom variants. A and B, Effect of BFA on root hydrotropic response of a GNOM BFA-resistant line. Seedlings expressing BFA-resistant GNOM (GN^M696L) were subjected to hydrotropic assay together with those of wild type (Col). Seedlings were treated with BFA at a concentration of 10⁻⁵ m, and hydrotropism was compared to mock-treated seedlings. Photomicrographs (A) and curvature (B) were collected 12 h after hydrotropic stimulation. Data in B represent the means ± sd of 16 individuals. White bar, Control roots of wild type; gray bar, control roots of GN^M696L; blue bar, BFA-treated roots of wild type; red bar, BFA-treated roots of GN^M696L. C and D, Hydrotropic response of a weak gnom allele, gnom^B/E. Seedlings of gnom^B/E were subjected to hydrotropic assay together with those of wild type (Col). Photomicrographs (C) and curvature (D) were collected as described above. Data in D represent the means ± sd of 28 and 23 for Col and gnom^B/E individuals, respectively. White bar, wild type; green bar, gnom^B/E. In A and C, the arrow (g) indicates the direction of the gravitational force.