Figure 2.

Absorbance patterns for mature seeds of rice, barley, and coffee. A, Comparison of absorbance patterns for pigment extracts from wild type (WT) or mutant before or after heat stress. B, Fluorescence intensity under UV light for samples in A. C, Comparison of absorbance patterns for extracts from rice, barley, and coffee. D, Fluorescence intensity under UV light for samples in C. E, Measurement of Amadori products of WT and mutant seeds. Seed proteins (200 μg) isolated from stored seeds were used for the test. The protein solution was mixed with nitro blue tetrazolium reagent. After incubation at 42°C for 10 to 20 min, absorbance was measured at 550 nm.