Table IV.
Secretory proteins encoded by anther-sporophytic-specific transcripts of progressive developmental stages
Transcripts were selected for their TPM >5× those in other organs (see legends to Tables I and III). They were further chosen for encoding proteins present outside the cell (probability ≥0.5 PSORT score). These transcripts are categorized according to their TPM of ≥10, 100 and 1,000, and their percentages in the total transcripts are shown. Transcripts encoding outside proteins in MPSS transcriptomes of leaves, roots, and ovaries are included for comparison. The lower portion summarizes groupings of the most abundant secretory proteins according to their possible functions. A list of all the outside proteins from transcripts of TPM ≥100 of each developmental stage is provided in Table S3.
| SBS
|
MPSS
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Developing Anthers
|
Pollen | Mature Leaf | Mature Root | Ovary and Stigma | ||||||
| Meiosis | Tetrad | Early Vacuolated | Late Vacuolated | Mitosis | Mature Pollen | |||||
| No. of transcripts (% transcripts encoding exterior proteins) | ||||||||||
| Transcripts | ||||||||||
| TPM ≥10 | 1,403 (7.1) | 933 (7.6) | 1,147 (8.3) | 885 (5.8) | 426 (5.2) | 200 (4.5) | 437 (10.5) | 195 (6.7) | 594 (12.5) | 367 (5.2) |
| TPM ≥100 | 247 (12.6) | 248 (12.5) | 288 (11.5) | 262 (7.3) | 89 (9.0) | 31 (9.7) | 247 (13.8) | 78 (2.6) | 152 (10.5) | 141 (6.4) |
| TPM ≥1,000 | 22 (31.8) | 33 (27.3) | 28 (39.3) | 26 (15.4) | 13 (23.1) | 4 (0.0) | 83 (15.7) | 21 (4.8) | 15 (20.0) | 15 (20.0) |
| Exterior proteins encoded by transcripts with: | TPM ≥100 | TPM ≥1,000 | ||||||||
| Total in all stages | 73 | 25 | ||||||||
| Lipid transfer/related proteins | 9 (12.3%) | 8 (32.0%) | ||||||||
| Unknown/hypothetical proteins | 25 (34.3%) | 7 (28.0%) | ||||||||
| Hydrolases (including wall-related) | 20 (27.4%) | 7 (28.0%) | ||||||||
| Others | 19 (26.0%) | 3 (12.0%) | ||||||||