Table I.
Characterization of enzyme activities in roots of 5-week-old FL and mMDH antisense transgenic plants
Values are presented as means ± se of six individual plants per line; values shown in boldface were determined by the t test to be significantly different (P < 0.05) from the wild type. –, Not determined.
| Enzyme Activities | Wild Type | mMDH7 | mMDH8 | mMDH21 | FL11 | FL41 | FL63 |
|---|---|---|---|---|---|---|---|
| μmol g−1 fresh weight min−1 | |||||||
| Total MDH-NAD dependent | 3,420 ± 310 | 1,540 ± 140 | 1,330 ± 140 | 1,780 ± 160 | – | – | – |
| Fumarase | 40.2 ± 5.1 | – | – | – | 8.72 ± 0.9 | 7.12 ± 0.9 | 22.5 ± 2.1 |