Table II.
Effects of decreased fumarase and mMDH activity on sugars, starch, and protein contents of 5-week-old mature antisense root tips
Data are presented as means ± se of six individual plants per line. Values in boldface were determined by the t test to be significantly different (P < 0.05) from the wild type. Values are presented as μmol g−1 fresh weight, except for protein, which is μg g−1 fresh weight.
| Sugars/Protein | Wild Type | mMDH7 | mMDH8 | mMDH21 | FL11 | FL41 | FL63 |
|---|---|---|---|---|---|---|---|
| Glc | 48.6 ± 6.7 | 35.2 ± 5.6 | 48.9 ± 4.7 | 33.1 ± 2.5 | 31.0 ± 2.1 | 35.0 ± 5.9 | 29.5 ± 4.1 |
| Fru | 38.1 ± 0.5 | 43.4 ± 2.7 | 40.6 ± 5.9 | 36.9 ± 7.4 | 29.0 ± 2.7 | 34.2 ± 1.0 | 24.3 ± 5.2 |
| Suc | 11.6 ± 1.9 | 15.0 ± 2.3 | 14.2 ± 2.1 | 12.7 ± 1.0 | 11.3 ± 1.2 | 9.9 ± 1.6 | 5.5 ± 1.1 |
| Total soluble sugars (hexose equivalents) | 89.0 ± 18.4 | 90.5 ± 6.0 | 104.9 ± 7.4 | 72.1 ± 5.7 | 72.7 ± 4.3 | 74.2 ± 5.8 | 54.0 ± 5.5 |
| Starch | 15.1 ± 2.7 | 6.7 ± 1.6 | 8.2 ± 0.7 | 4.7 ± 0.7 | 8.8 ± 1.1 | 9.1 ± 0.9 | 12.6 ± 1.7 |
| Protein | 147 ± 15 | 196 ± 54 | 152 ± 53 | 129 ± 13 | 198 ± 53 | 124 ± 6 | 187 ± 21 |