Table III.
Relative metabolite contents of roots from mMDH and FL lines
Metabolites were determined as described in “Materials and Methods.” Data are normalized with respect to mean response calculated for the wild type (to allow statistical assessment in the same way). Values are presented as means ± se of six individual plants per line, and values in boldface were determined by t test to be significantly different (P < 0.05) from the wild type.
| Metabolite | Wild Type | mMDH7 | mMDH8 | mMDH21 | FL11 | FL41 | FL63 |
|---|---|---|---|---|---|---|---|
| Aconitate | 1.00 ± 0.48 | 0.99 ± 0.35 | 0.87 ± 0.40 | 0.88 ± 0.39 | 0.56 ± 0.52 | 0.24 ± 0.35 | 0.76 ± 0.73 |
| Ala | 1.00 ± 0.30 | 1.05 ± 0.22 | 1.76 ± 0.41 | 1.22 ± 0.26 | 1.24 ± 0.26 | 2.61 ± 0.23 | 1.29 ± 0.17 |
| Ara | 1.00 ± 0.33 | 2.66 ± 0.33 | 2.91 ± 0.28 | 1.51 ± 0.39 | 1.22 ± 0.31 | 1.96 ± 0.44 | 0.83 ± 0.25 |
| Arg | 1.00 ± 0.36 | 0.92 ± 0.46 | 1.71 ± 0.49 | 0.97 ± 0.60 | 0.69 ± 0.56 | 1.54 ± 0.60 | 0.85 ± 0.60 |
| Asn | 1.00 ± 0.38 | 1.43 ± 0.30 | 1.55 ± 0.86 | 0.80 ± 0.48 | 0.53 ± 0.71 | 0.42 ± 0.60 | 0.51 ± 0.85 |
| Asp | 1.00 ± 0.47 | 0.85 ± 0.58 | 0.58 ± 0.31 | 0.41 ± 0.49 | 1.98 ± 0.50 | 0.67 ± 0.27 | 0.45 ± 0.39 |
| Citrate | 1.00 ± 0.32 | 1.43 ± 0.30 | 1.75 ± 0.31 | 0.69 ± 0.38 | 0.47 ± 0.26 | 0.23 ± 0.49 | 0.29 ± 0.48 |
| Cys | 1.00 ± 0.17 | 2.43 ± 0.44 | 2.84 ± 0.39 | 1.20 ± 0.35 | 1.31 ± 0.31 | 0.90 ± 0.25 | 0.78 ± 0.11 |
| Dehydroascorbate | 1.00 ± 0.33 | 1.25 ± 0.35 | 1.44 ± 0.53 | 0.94 ± 0.38 | 0.47 ± 0.29 | 0.64 ± 0.76 | 0.21 ± 0.24 |
| Fru | 1.00 ± 0.36 | 0.78 ± 0.55 | 1.55 ± 0.42 | 0.77 ± 0.26 | 0.17 ± 0.40 | 0.08 ± 0.53 | 0.17 ± 0.35 |
| Fru 6-P | 1.00 ± 0.47 | 1.03 ± 0.30 | 1.06 ± 0.32 | 2.03 ± 0.47 | 1.62 ± 0.33 | 0.73 ± 0.77 | 1.16 ± 0.69 |
| Fumarate | 1.00 ± 0.23 | 1.97 ± 0.58 | 2.08 ± 0.56 | 0.54 ± 0.25 | 2.19 ± 0.47 | 0.70 ± 0.30 | 0.63 ± 0.38 |
| GABA | 1.00 ± 0.21 | 1.13 ± 0.37 | 1.38 ± 0.40 | 0.85 ± 0.38 | 0.29 ± 0.22 | 0.42 ± 0.36 | 0.25 ± 0.21 |
| Gal | 1.00 ± 0.31 | 2.04 ± 0.44 | 1.25 ± 0.50 | 1.75 ± 0.57 | 0.32 ± 0.32 | 0.40 ± 0.57 | 0.20 ± 0.44 |
| Galacturonate | 1.00 ± 0.51 | 1.42 ± 0.49 | 1.87 ± 0.56 | 1.11 ± 0.49 | 1.04 ± 0.71 | 0.27 ± 0.58 | 0.87 ± 0.85 |
| Glc | 1.00 ± 0.32 | 1.27 ± 0.38 | 0.92 ± 0.48 | 1.64 ± 0.54 | 0.47 ± 0.28 | 0.26 ± 0.41 | 0.25 ± 0.40 |
| Glc 6-P | 1.00 ± 0.41 | 0.84 ± 0.22 | 1.00 ± 0.38 | 1.29 ± 0.42 | 1.50 ± 0.38 | 0.42 ± 0.76 | 1.09 ± 0.80 |
| Glu | 1.00 ± 0.41 | 0.87 ± 0.45 | 1.18 ± 0.32 | 0.97 ± 0.49 | 0.26 ± 0.42 | 0.16 ± 0.26 | 0.13 ± 0.47 |
| Gln | 1.00 ± 0.33 | 5.66 ± 0.41 | 10.49 ± 0.61 | 6.95 ± 0.54 | 0.10 ± 0.20 | 0.20 ± 0.43 | 0.06 ± 0.19 |
| Glyceric 3-P | 1.00 ± 0.25 | 0.62 ± 0.29 | 0.28 ± 0.25 | 0.35 ± 0.47 | 0.15 ± 0.31 | 0.37 ± 0.67 | 0.13 ± 0.18 |
| Glycerol 3-P | 1.00 ± 0.30 | 1.54 ± 0.25 | 1.80 ± 0.41 | 1.68 ± 0.56 | 2.36 ± 0.41 | 2.72 ± 0.22 | 0.56 ± 0.37 |
| Gly | 1.00 ± 0.13 | 2.31 ± 0.44 | 0.80 ± 0.23 | 0.70 ± 0.22 | 1.46 ± 0.18 | 0.45 ± 0.23 | 0.56 ± 0.32 |
| Isocitrate | 1.00 ± 0.27 | 1.92 ± 0.35 | 1.27 ± 0.32 | 1.17 ± 0.37 | 1.10 ± 0.49 | 0.30 ± 0.44 | 0.98 ± 0.67 |
| Ile | 1.00 ± 0.24 | 1.46 ± 0.24 | 1.55 ± 0.55 | 1.87 ± 0.52 | 1.23 ± 0.48 | 0.65 ± 0.25 | 0.83 ± 0.29 |
| l-Ascorbate | 1.00 ± 0.36 | 1.95 ± 0.53 | 1.22 ± 0.32 | 0.80 ± 0.39 | 0.64 ± 0.51 | 0.29 ± 0.33 | 0.25 ± 0.55 |
| Leu | 1.00 ± 0.31 | 1.15 ± 0.49 | 1.84 ± 0.45 | 0.72 ± 0.41 | 0.89 ± 0.46 | 0.74 ± 0.66 | 0.58 ± 0.29 |
| Lys | 1.00 ± 0.28 | 3.65 ± 0.62 | 4.26 ± 0.62 | 2.97 ± 0.39 | 1.50 ± 0.39 | 1.17 ± 0.58 | 1.20 ± 0.47 |
| Malate | 1.00 ± 0.20 | 0.94 ± 0.59 | 1.03 ± 0.27 | 0.59 ± 0.33 | 0.21 ± 0.36 | 0.13 ± 0.33 | 0.37 ± 0.32 |
| Maltose | 1.00 ± 0.36 | 2.42 ± 0.22 | 1.90 ± 0.26 | 2.15 ± 0.30 | 1.65 ± 0.27 | 0.88 ± 0.53 | 0.58 ± 0.28 |
| Mannitol | 1.00 ± 0.32 | 1.54 ± 0.34 | 1.78 ± 0.31 | 1.39 ± 0.37 | 2.13 ± 0.64 | 4.60 ± 0.90 | 8.79 ± 0.66 |
| Met | 1.00 ± 0.38 | 0.58 ± 0.34 | 0.16 ± 0.28 | 0.45 ± 0.39 | 0.15 ± 0.39 | 0.10 ± 0.48 | 0.07 ± 0.30 |
| Myoinositol | 1.00 ± 0.40 | 0.70 ± 0.30 | 0.45 ± 0.28 | 0.38 ± 0.33 | 0.27 ± 0.30 | 0.28 ± 0.48 | 0.12 ± 0.21 |
| Palmeate (16:0) | 1.00 ± 0.35 | 2.15 ± 0.38 | 1.58 ± 0.40 | 1.17 ± 0.42 | 0.88 ± 0.32 | 0.49 ± 0.27 | 0.49 ± 0.38 |
| Phe | 1.00 ± 0.30 | 1.65 ± 0.41 | 1.33 ± 0.48 | 0.85 ± 0.51 | 0.41 ± 0.48 | 0.46 ± 0.50 | 0.33 ± 0.35 |
| Pro | 1.00 ± 0.35 | 1.55 ± 0.55 | 1.11 ± 0.42 | 1.21 ± 0.65 | 1.02 ± 0.50 | 0.19 ± 0.23 | 0.81 ± 0.29 |
| Putrescine | 1.00 ± 0.30 | 2.78 ± 0.30 | 1.31 ± 0.42 | 3.24 ± 0.38 | 1.07 ± 0.27 | 1.05 ± 0.64 | 0.68 ± 0.33 |
| Quinate | 1.00 ± 0.36 | 0.78 ± 0.38 | 1.34 ± 0.30 | 1.45 ± 0.62 | 1.05 ± 0.33 | 0.60 ± 0.37 | 0.39 ± 0.27 |
| Saccharate | 1.00 ± 0.28 | 0.66 ± 0.27 | 0.61 ± 0.40 | 0.73 ± 0.30 | 0.71 ± 0.21 | 0.44 ± 0.52 | 0.17 ± 0.18 |
| Ser | 1.00 ± 0.53 | 2.20 ± 0.39 | 1.35 ± 0.49 | 0.97 ± 0.48 | 0.69 ± 0.39 | 0.64 ± 0.64 | 0.49 ± 0.21 |
| Shikimate | 1.00 ± 0.30 | 3.25 ± 0.31 | 1.01 ± 0.39 | 0.86 ± 0.24 | 0.87 ± 0.38 | 0.17 ± 0.30 | 0.25 ± 0.39 |
| Stearate (18:0) | 1.00 ± 0.33 | 6.67 ± 0.42 | 7.45 ± 0.41 | 5.15 ± 0.45 | 6.53 ± 0.27 | 1.99 ± 0.19 | 1.55 ± 0.38 |
| Succinate | 1.00 ± 0.32 | 3.68 ± 0.30 | 8.60 ± 0.31 | 8.27 ± 0.37 | 3.31 ± 0.38 | 5.74 ± 0.58 | 7.09 ± 0.24 |
| Suc | 1.00 ± 0.30 | 2.04 ± 0.44 | 1.78 ± 0.40 | 1.09 ± 0.26 | 0.92 ± 0.68 | 0.26 ± 0.36 | 0.21 ± 0.33 |
| Threonate | 1.00 ± 0.56 | 0.96 ± 0.36 | 0.81 ± 0.37 | 0.49 ± 0.35 | 0.46 ± 0.29 | 0.24 ± 0.11 | 0.31 ± 0.10 |
| Thr | 1.00 ± 0.31 | 2.45 ± 0.39 | 0.82 ± 0.20 | 1.32 ± 0.58 | 1.11 ± 0.30 | 0.71 ± 0.30 | 0.93 ± 0.35 |
| Trehalose | 1.00 ± 0.33 | 1.34 ± 0.29 | 2.25 ± 0.45 | 1.29 ± 0.36 | 1.40 ± 0.28 | 0.59 ± 0.63 | 0.58 ± 0.43 |
| Trp | 1.00 ± 0.34 | 3.13 ± 0.27 | 3.77 ± 0.53 | 1.69 ± 0.68 | 0.83 ± 0.45 | 1.27 ± 0.60 | 2.13 ± 0.50 |
| Tyramine | 1.00 ± 0.35 | 6.54 ± 0.35 | 2.76 ± 0.49 | 3.40 ± 0.50 | 1.05 ± 0.52 | 0.86 ± 0.53 | 0.56 ± 0.28 |
| Tyr | 1.00 ± 0.28 | 3.31 ± 0.35 | 5.28 ± 0.68 | 0.94 ± 0.16 | 0.91 ± 0.66 | 1.34 ± 0.67 | 1.21 ± 0.77 |
| Val | 1.00 ± 0.20 | 0.96 ± 0.23 | 1.34 ± 0.46 | 0.73 ± 0.31 | 1.05 ± 0.35 | 1.02 ± 0.56 | 0.64 ± 0.22 |
| Xyl | 1.00 ± 0.39 | 8.93 ± 0.42 | 2.08 ± 0.28 | 2.46 ± 0.33 | 1.67 ± 0.56 | 1.43 ± 0.62 | 0.58 ± 0.30 |