Table V.
Metabolite profiles of grafting combinations of transgenic scion with wild-type rootstock (MDH:WT, FL:WT), wild-type scion with transgenic rootstock (WT:MDH, WT:FL), wild-type self-grafting (WT:WT), or ungrafted entities (wild type, MDH, FL)
Data are normalized with respect to the mean response calculated for the wild type. Values presented are means ± se of four replicates; values in boldface were determined by the t test to be significantly different (P < 0.05). nd, Not detected.
| Metabolite | Wild Type | mMDH8 | FL41 | MDH:WT | FL:WT | WT:MDH | WT:FL | WT:WT |
|---|---|---|---|---|---|---|---|---|
| Ala | 1.00 ± 0.32 | 1.08 ± 0.27 | 1.81 ± 0.24 | 0.81 ± 0.33 | 0.38 ± 0.13 | 0.85 ± 0.10 | 2.29 ± 0.89 | 0.88 ± 0.26 |
| β-Ala | 1.00 ± 0.09 | 1.89 ± 0.36 | 3.30 ± 0.17 | 2.62 ± 1.15 | 0.62 ± 0.10 | 0.85 ± 0.09 | 1.12 ± 0.16 | 1.05 ± 0.23 |
| Asn | 1.00 ± 0.55 | 1.44 ± 0.33 | 2.64 ± 0.23 | 0.46 ± 0.21 | 0.94 ± 0.46 | 0.97 ± 0.66 | 2.80 ± 0.60 | 0.33 ± 0.11 |
| Asp | 1.00 ± 0.17 | 1.39 ± 0.30 | 2.96 ± 0.18 | 0.52 ± 0.22 | 1.04 ± 0.15 | 0.90 ± 0.19 | 1.18 ± 0.01 | 0.82 ± 0.16 |
| Benzoate | 1.00 ± 0.14 | 0.79 ± 0.17 | 1.77 ± 0.14 | 0.62 ± 0.10 | 0.82 ± 0.11 | 0.86 ± 0.05 | 1.04 ± 0.12 | 0.97 ± 0.13 |
| GABA | 1.00 ± 0.29 | 0.76 ± 0.34 | 1.76 ± 0.35 | 0.97 ± 0.29 | 1.20 ± 0.19 | 1.39 ± 0.21 | 1.61 ± 0.12 | 1.10 ± 0.24 |
| Citrate | 1.00 ± 0.19 | 1.06 ± 0.31 | 2.76 ± 0.21 | 0.13 ± 0.02 | 1.07 ± 0.07 | 0.73 ± 0.19 | 0.74 ± 0.25 | 0.72 ± 0.22 |
| Fru | 1.00 ± 0.02 | 0.87 ± 0.30 | 1.30 ± 0.23 | 0.07 ± 0.03 | 0.88 ± 0.07 | 0.82 ± 0.17 | 0.90 ± 0.03 | 1.02 ± 0.02 |
| Fru 6-P | 1.00 ± 0.31 | 1.02 ± 0.42 | 2.49 ± 0.45 | 2.12 ± 0.95 | 1.04 ± 0.12 | 0.74 ± 0.19 | 1.55 ± 0.21 | 0.75 ± 0.12 |
| Fumarate | 1.00 ± 0.09 | 1.21 ± 0.22 | 2.31 ± 0.19 | 2.12 ± 0.94 | 0.58 ± 0.11 | 0.49 ± 0.10 | 0.82 ± 0.11 | 1.04 ± 0.38 |
| Galacturonate | 1.00 ± 0.26 | 0.97 ± 0.51 | 3.05 ± 0.52 | 0.68 ± 0.30 | 1.39 ± 0.06 | 1.22 ± 0.16 | 1.89 ± 0.22 | 1.14 ± 0.19 |
| Glc | 1.00 ± 0.65 | 0.79 ± 0.27 | 2.35 ± 0.26 | 0.08 ± 0.04 | 3.95 ± 0.83 | 0.58 ± 0.54 | 0.79 ± 0.53 | 1.56 ± 0.56 |
| Glc 6-P | 1.00 ± 0.29 | 1.07 ± 0.41 | 2.39 ± 0.42 | 2.05 ± 0.91 | 1.03 ± 0.10 | 0.76 ± 0.20 | 1.66 ± 0.22 | 0.73 ± 0.14 |
| Gln | 1.00 ± 0.24 | 0.90 ± 0.35 | 4.31 ± 0.24 | 0.09 ± 0.04 | 1.06 ± 0.23 | 1.07 ± 0.25 | 1.44 ± 0.01 | 0.85 ± 0.25 |
| Glycerate | 1.00 ± 0.15 | 1.26 ± 0.23 | 2.27 ± 0.16 | 2.81 ± 1.22 | 1.18 ± 0.14 | 1.32 ± 0.20 | 2.09 ± 0.24 | 1.07 ± 0.24 |
| Glycerol | 1.00 ± 0.20 | 1.11 ± 0.19 | 2.80 ± 0.11 | 2.15 ± 0.78 | 1.25 ± 0.30 | 2.19 ± 0.53 | 2.72 ± 0.72 | 2.47 ± 0.48 |
| Glycerol 3-P | 1.00 ± 0.27 | 1.30 ± 0.32 | 2.31 ± 0.25 | 1.00 ± 0.44 | 1.38 ± 0.23 | 2.22 ± 0.78 | 4.05 ± 1.19 | 2.73 ± 0.91 |
| Gly | 1.00 ± 0.12 | 1.62 ± 0.30 | 3.17 ± 0.12 | 0.76 ± 0.31 | 1.17 ± 0.17 | 1.12 ± 0.12 | 1.67 ± 0.41 | 1.52 ± 0.54 |
| His | 1.00 ± 0.27 | nd | nd | 0.26 ± 0.12 | 0.89 ± 0.20 | 0.90 ±.0.45 | 2.17 ± 0.32 | 1.98 ± 1.04 |
| Myoinositol | 1.00 ± 0.10 | 0.64 ± 0.48 | 1.83 ± 0.38 | 0.56 ± 0.21 | 1.13 ± 0.03 | 1.14 ± 0.02 | 1.09 ± 0.01 | 0.82 ± 0.12 |
| Ile | 1.00 ± 0.16 | 1.19 ± 0.36 | 2.04 ± 0.20 | 0.90 ± 0.38 | 1.67 ± 0.24 | 1.91 ± 0.83 | 2.33 ± 0.58 | 1.34 ± 0.36 |
| Lys | 1.00 ± 0.18 | 0.78 ± 0.51 | 1.66 ± 0.44 | 0.64 ± 0.27 | 1.14 ± 0.19 | 1.01 ± 0.27 | 1.69 ± 0.18 | 1.01 ± 0.21 |
| Malate | 1.00 ± 0.19 | 1.37 ± 0.31 | 2.55 ± 0.32 | 0.57 ± 0.20 | 1.05 ± 0.08 | 0.86 ± 0.21 | 1.19 ± 0.01 | 0.93 ± 0.11 |
| Maltitol | 1.00 ± 0.25 | 1.38 ± 0.25 | 3.37 ± 0.37 | 1.75 ± 0.77 | 1.94 ± 0.26 | 1.29 ± 0.24 | 2.31 ± 0.25 | 1.38 ± 0.44 |
| Maltose | 1.00 ± 0.35 | 1.36 ± 0.36 | 2.04 ± 0.27 | 2.17 ± 0.90 | 1.41 ± 0.20 | 1.08 ± 0.18 | 1.94 ± 0.30 | 1.06 ± 0.14 |
| Man | 1.00 ± 0.09 | 1.00 ± 0.19 | 2.63 ± 0.30 | 0.03 ± 0.01 | 1.15 ± 0.13 | 0.92 ± 0.22 | 1.79 ± 0.35 | 1.10 ± 0.17 |
| Met | 1.00 ± 0.25 | 1.03 ± 0.27 | 1.99 ± 0.27 | 0.55 ± 0.23 | 1.02 ± 0.24 | 0.86 ± 0.25 | 1.90 ± 0.26 | 1.11 ± 0.32 |
| Phe | 1.00 ± 0.21 | 1.03 ± 0.26 | 1.93 ± 0.24 | 0.73 ± 0.31 | 1.15 ± 0.14 | 1.05 ± 0.23 | 1.65 ± 0.20 | 1.19 ± 0.31 |
| Pro | 1.00 ± 0.15 | 2.15 ± 0.34 | 2.23 ± 0.34 | 1.99 ± 0.99 | 1.05 ± 0.15 | 0.93 ± 0.25 | 1.31 ± 0.24 | 0.76 ± 0.13 |
| Putrescine | 1.00 ± 0.12 | 0.67 ± 0.38 | 2.44 ± 0.46 | 0.17 ± 0.07 | 0.36 ± 0.07 | 0.69 ± 0.12 | 0.51 ± 0.06 | 0.92 ± 0.24 |
| Raffinose | 1.00 ± 0.38 | 3.62 ± 0.39 | 1.95 ± 0.23 | 0.18 ± 0.08 | 0.27 ± 0.02 | 0.25 ± 0.03 | 0.34 ± 0.05 | 0.25 ± 0.05 |
| Saccharate | 1.00 ± 0.20 | 0.83 ± 0.23 | 2.15 ± 0.21 | 0.62 ± 0.25 | 1.20 ± 0.03 | 1.23 ± 0.04 | 1.04 ± 0.04 | 1.12 ± 0.08 |
| Ser | 1.00 ± 0.43 | 2.25 ± 0.42 | 2.76 ± 0.24 | 1.20 ± 0.50 | 0.63 ± 0.08 | 0.58 ± 0.28 | 1.23 ± 0.29 | 0.60 ± 0.14 |
| Succinate | 1.00 ± 0.17 | 2.42 ± 0.29 | 1.20 ± 0.23 | 2.93 ± 1.27 | 0.88 ± 0.08 | 1.21 ± 0.11 | 1.08 ± 0.15 | 0.99 ± 0.17 |
| Suc | 1.00 ± 0.33 | 2.30 ± 0.56 | 1.42 ± 0.41 | nd | 0.80 ± 0.21 | 0.71 ± 0.15 | 1.50 ± 0.17 | 0.84 ± 0.28 |
| Thr | 1.00 ± 0.49 | 1.82 ± 0.37 | 2.36 ± 0.23 | 0.75 ± 0.31 | 0.78 ± 0.11 | 0.54 ± 0.17 | 0.85 ± 0.11 | 0.58 ± 0.09 |
| Threonate | 1.00 ± 0.49 | 0.72 ± 0.20 | 1.92 ± 0.26 | 0.75 ± 0.31 | 0.78 ± 0.11 | 0.54 ± 0.17 | 0.85 ± 0.11 | 0.58 ± 0.09 |
| Tyr | 1.00 ± 0.26 | 0.71 ± 0.46 | 1.52 ± 0.43 | 0.17 ± 0.07 | 1.00 ± 0.29 | 1.13 ± 0.21 | 1.38 ± 0.39 | 1.94 ± 0.48 |
| Trehalose | 1.00 ± 0.13 | 0.51 ± 0.42 | 1.59 ± 0.19 | 0.29 ± 0.13 | 0.68 ± 0.04 | 0.81 ± 0.16 | 0.99 ± 0.23 | 1.12 ± 0.28 |
| Trp | 1.00 ± 0.17 | 0.90 ± 0.49 | 1.34 ± 0.53 | 0.01 ± 0.00 | 1.07 ± 0.29 | 0.78 ± 0.12 | 0.60 ± 0.05 | 0.64 ± 0.15 |
| Val | 1.00 ± 0.10 | 1.12 ± 0.30 | 1.90 ± 0.18 | 0.91 ± 0.38 | 1.04 ± 0.17 | 0.95 ± 0.20 | 1.43 ± 0.17 | 1.21 ± 0.26 |
| Xylitol | 1.00 ± 0.22 | 1.06 ± 0.31 | 2.41 ± 0.26 | 0.16 ± 0.05 | 0.88 ± 0.11 | 1.13 ± 0.20 | 1.83 ± 0.46 | 1.74 ± 0.54 |