Figure 3.
Lysis of the human GBM cell line U87MG EGFR by EGFRvIII-specific MAb Y10 in the presence or absence of human macrophages. U87MG EGFR is a human GBM cell line transfected to express human EGFRvIII. Y10 is a murine IgG2a MAb that specifically recognizes EGFRvIII. Rabbit anti-EGFRvIII is a polyclonal affinity purified antiserum that specifically recognizes EGFRvIII and does not cross react with wild type EGFR. M221 is an isotype-matched control murine MAb and the rabbit control antibody is an affinity purified rabbit polyclonal antibody to an unrelated antigen. EGFRvIII specific antibodies were incubated for 72 hours with 3H thymidine labeled target cells with and without human macrophages. Antibodies were used at a concentration of 0.1 mg/mL and the macrophages were at an effector to target ratio of 100:1. Y10 produced a significantly greater lysis than M22.1 (P=0.0002).