Soluble amino acid content (+SEM) in the third youngest leaf at seven days after treatment with 500 µg jasmonic acid to the roots (RJA), to the shoot (SJA) or with acidic water (CON). A) Major amino acids. Letters over the bars indicate significant differences between total amino acid levels between treatment groups after ANOVA (Tukey unequal N HSD, p < 0.05). Grey: Thr; hatched: Arg; black: Iso/Leu; crossed: Pro; white: total of minor amino acids. B) Minor amino acids, separated in four groups. From left to right: Group 1 (white: His): significant increase in SJA only; Group 2 (hatched: Gln): significant decrease in SJA only; Group 3 (grey: Ser, crossed: Lys, chequered: Tyr; black: Met; dark grey: Phe): significant decrease in both JA treatments; Group 4 (chequered: Asp, grey hatched: Asn, dotted: Glu; hatched: Trp): no significant differences between treatments.