Figure 4.

High resolution Z-stack confocal micrographs of a sycamore cultured cell after two hours incubation in incubation medium containing 10¹⁴ CdSe/ZnS quantum dots per ml and 1 mg ml⁻¹ d-TR. Cell was observed using an excitation wavelength of 488 nm and emission window of 500–540 nm for CdSe/ZnS fluorescence (A); 543 nm excitation and emission window of 560–700 nm for Texas-Red (B); and white light (C). (D) is a superimposed image of (A–C) showing the colocalization of CdSe/ZnS quantum dots and d-TR in various contiguous vesicles (encircled).