Figure 11.

RT-PCR analysis of inducible expression of ABP1 and ABP4 in maize mesocotyls. 307 and 3394 seedlings were grown in vitro on BM in D, R or FR. 5-d-old maize seedlings were used to isolate RNA. One µg of total RNA was used to synthesize cDNA. The synthesized cDNA was used to amplify maize ABP1 (403 bp) and ABP4 (403 bp) gene transcripts. In (A) the lowest line is a loading control showing ethidium bromide-stained rRNA. (B and C) show quantification of the expression data (mean ± SE) of three independent experiments for gene ABP1 and ABP4, respectively.