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. 2008 Jul-Sep;2(3):161–166. doi: 10.4161/cam.2.3.6549

Figure 3.

Figure 3

(A) Pervanadate induces extensive tyrosine phosphorylation of cellular proteins. Equally loaded immunoblots of whole cell extracts untreated (left) and pervanadate-treated (right) MDCK cells with anti-phosphotyrosine antibody PY99. (B) Pervanadate causes tyrosine phosphorylation of Dsg2 and Pg, which co-precipitate from the NP-40-soluble fraction. Cultures were either untreated or treated with pervanadate for 15 minutes prior to detergent fractionation (S = soluble; I = insoluble) and subsequent immunoprecipitation of ectopic mDsg2HA. Samples were fractionated by SDS-PAGE and immunoblotted with an anti-phosphotyrosine antibody. The membrane was then sequentially re-probed for mDsg2HA and Pg.