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. 2008 Jul-Sep;2(3):161–166. doi: 10.4161/cam.2.3.6549

Figure 4.

Figure 4

Pervanadate treatment reduces the amount of Dsg2 in the soluble fraction. Confluent cultures of untransfected MDCK cells were untreated, treated with pervanadate for 15 mins (PV15) or treated with 50 nM phorbol myristate acetate for 30 (PMA 30) or 60 minutes (PMA 60). Following the treatments the cells were fractioned into NP-40-soluble and -insoluble fractions and equal quantities of protein subjected to SDS-PAGE and immunoblotting for Dsg2. PMA treatment did not alter the level of Dsg2 in either of the detergent fractions whereas pervanadate treatment reduced the amount of Dsg2 in the soluble fraction.