Table 3.
Differential TDFs amplified in the Saccharum spp .- G. diazotrophicus - X. albilineans interactions
| TDF1 | Size (bp) | Homology2 | Protein3 | E-value4 | G.d → X.a treatment5 | X.a → G.d treatment6 | ||||||
| T0 | T1 | T2 | T3 | T0 | T1 | T2 | T3 | |||||
| 1 | 661 | XP_468441 (O. sativa) | Ethylene receptor | 7e-42 | x | x | ||||||
| 2 | 653 | AAM00285 (O. sativa) | Transcription factor to ethylene response | 1e-50 | x | x | xx | x | ||||
| 3 | 613 | CAD56466 (T. aestivum) | Transcription factor to ethylene response | 3e-52 | x | x | ||||||
| 4 | 608 | AAF04899 (A. thaliana) | Induced protein by auxin - Aux/IAA | 1e-31 | x | x | ||||||
| 5 | 550 | AAA33401 (L.usitatissimum) | Ubiquitin | 1e-54 | x | xx | ||||||
| 6 | 544 | AAP92744 (O. sativa) | Transcription factor to ethylene response | 4e-06 | x | xx | x | |||||
| 7 | 543 | XP_466220 (O. sativa) | Transcription factor for auxin response - ARF1 | 1e-36 | x | x | xx | |||||
| 8 | 529 | - | - | - | x | x | x | |||||
| 9 | 508 | BAA19242 (S. officinarum) | Sucrose-phosphate synthase | 2e-60 | x | x | x | x | x | |||
| 10 | 505 | BAD82174 (O. sativa) | Ribosomal protein 60S | 4e-63 | x | x | ||||||
| 11 | 494 | BAD54446 (O. sativa) | Xiloglucan endotransglycosilase | 1e-42 | x | x | ||||||
| 12 | 487 | TC70637* | - | 4e-100* | x | x | xx | |||||
| 13 | 480 | XP_475484 (O. sativa) | Transcription factor to ethylene response | 4e-36 | x | xx | xxx | x | x | |||
| 14 | 472 | XP_468566 (O. sativa) | Transcription factor BTF3 | 3e-27 | x | x | ||||||
| 15 | 444 | BU925674* | - | 1e-93* | x | x | ||||||
| 16 | 437 | TC61807* | - | 2e-89* | x | x | ||||||
| 17 | 436 | AAV98702 (O. sativa) | Transcription factor to ethylene response | 7e-59 | x | x | x | |||||
| 18 | 428 | - | - | - | x | xx | ||||||
| 19 | 424 | AAQ20898 (O. sativa) | Transcription factor to ethylene response | 5e-25 | x | x | x | |||||
| 20 | 420 | TC56550* | - | 1e-81* | x | x | ||||||
| 21 | 401 | AAQ06261 (S. bicolor) | β- 1,3 Glucanase | 8e-30 | x | x | x | |||||
| 22 | 391 | NP_176303 (A. thaliana) | - | 7e-05 | x | xx | ||||||
| 23 | 382 | - | - | - | x | x | x | |||||
| 24 | 373 | BAB84334 (O. sativa) | UDP- glucuronic acid descarboxilase | 4e-33 | x | x | x | x | ||||
| 25 | 371 | XP_481608 (O. sativa) | Endo-ribonuclease | 8e-18 | x | x | x | x | ||||
| 26 | 368 | - | - | - | x | xx | xx | x | x | |||
| 27 | 359 | XP_482965 (O. sativa) | F0 ATP synthase from Mitocondria | 8e-31 | x | x | x | |||||
| 28 | 357 | XP_550382 (O. sativa) | Induced protein by auxin - Aux/IAA | 3e-32 | x | xx | ||||||
| 29 | 354 | AAP53941 (O. sativa) | Transcription factor to ethylene response | 4e-21 | x | x | x | xxx | ||||
| 30 | 341 | XP_468441 (O. sativa) | Ethylene receptor | 1e-13 | x | x | x | |||||
| 31 | 325 | TC50195* | - | 1e-65 | x | x | x | x | ||||
| 32 | 318 | AAG33924 (R. pseudoacacia) | Protein regulated by auxin | 8e-15 | x | x | x | x | ||||
| 33 | 309 | AAT38997 (B. vulgaris) | Protein ligating of RNA | 7e-29 | x | x | x | |||||
| 34 | 305 | XP_479969 (O. sativa) | β- 1,3 Glucanase | 2e-32 | x | x | ||||||
| 35 | 293 | XP_550431 (O. sativa) | Glutamine tRNA synthetase | 2e-16 | x | x | x | x | ||||
| 36 | 291 | AAX95628 (O. sativa) | Transposable element | 3e-05 | x | xx | x | x | ||||
| 37 | 288 | XP_476644 (O. sativa) | β- 1,3 Glucanase | 9e-16 | x | x | x | |||||
| 38 | 260 | XP_475484 (O. sativa) | Transcription factor to ethylene response | 1e-19 | x | xx | xxx | x | x | |||
| 39 | 251 | AAT85197 (O. sativa) | - | 1e-20 | x | x | x | x | ||||
| 40 | 245 | TC48467* | - | 2.4e-48 | x | x | x | x | x | |||
| 41 | 243 | AAP53387 (O. sativa) | Transcription factor to ethylene response | 1e-18 | x | x | x | |||||
| 42 | 243 | CAG30776 (E. globulus) | Ubiquitin | 3e-20 | x | x | x | x | x | |||
| 43 | 241 | AAP83936 (G. hirsutum) | Transcription factor DREB | 1e-07 | x | xx | ||||||
| 44 | 237 | AAF40112 (O. sativa) | Photomorphogenic element | 4e-05 | x | x | ||||||
| 45 | 232 | XP_467394 (O. sativa) | Response element to ethylene | 5e-14 | x | xx | x | |||||
| 46 | 232 | XP_483731 (O. sativa) | - | 5e-05 | x | x | x | x | ||||
| 47 | 227 | TC50866* | - | 1e-38* | x | x | ||||||
Transcript derived fragment (TDF) codices corresponding to polymorphism band in acrylamide gel.
Sequences were compared with those deposited in the protein database NCBI (BLASTX program), ESTs sugarcane database of TIGR Gene Indice (*), and ESTs database of NCBI () (TBLASTX program). Access number of protein, EST with significant homology and corresponding specie are showed.
Identification of homologue protein. Symbol “-” represent an EST without significative homology to protein with knows functions.
E-value alignment with the most homologue sequence in databases.
G.d→X.a treatment. Sugarcane plants inoculated firstly with G. diazotrophicus and after seven days with the pathogenic bacteria X. albilineas.
X.a → G.d treatment. Sugarcane plants inoculated firstly with the pathogen X. albilineans and after seven days with the symbiotic bacteria G. diazotrophicus.