Proof of CER6 (A), actin2 (B) and Ef1α (C) transcripts in Arabidopsis thaliana rosette leaves. a, positive control; b, directly after first de-waxing; c, directly after second de-waxing; d, 1 h after second de-waxing; e, 2 h after second de-waxing; f, 3 h after second de-waxing. CER6 fragment: 212 bp, actin2 fragment: 226 bp, eF1α fragment: 250 bp. Arrow points to 500 bp marker.