Figure 4.

Immunostaining of CER6 protein in protein extracts of Arabidopsis thaliana. (A) a: CER6 fragment (positive control); b protein from fully developed leaves, control; c: directly after second de-waxing; d: 2 h after second de-waxing; e: 4 h after second de-waxing; f,g: 17,5 h after second de-waxing. Arrow marks position of full length CER6 protein. Numbers indicate marker proteins (kDa). (B) controls; a: flower; b: stem; c: upper stem leaves; d: lower stem leaves; e: young rosette leaves. (C) samples from plants after 24 h exposure to 100/50 mg camphor/menthol, a–e same plant parts as shown in blot B.