Figure 1.

Capacitance measurement of cell concentrations in tissue constructs. (A) Capacitance at 0.4 MHz of fibroblasts cells at different concentrations in a fibrin gel, showing a linear relation between capacitance and cell concentration. (B) Constantly monitoring the capacitance at 0.4 MHz allows the cell concentration in the artificial tissue to be followed in time. The gel was inoculated with 100.000 human fibroblast cells/ml in DMEM growth medium with 10% fetal calf serum. Capacitance measurements were performed with an Aber Instruments model 220 Biomass Monitor (Aber Instruments, Aberystwyth, UK).