Figure 4.

Methylation of regulatory regions of imprinted genes in uniparental ES cells and their hematopoietic derivatives in recipients. Methylation analysis was performed by bisulfite sequencing.⁶⁷ Samples were taken from animals with a > 95% contribution (no detectable host or blastocyst component as per Gpi1 analysis) from the ES cell derived component. Circles represent the percentage of methylation detected in all clones (full: methylated, ¼, ½ or ¾: 25, 50 or 75% methylated, empty: not methylated; approximation of methylation to nearest %). Rec., recipient; BM, bone marrow (cell type analyzed for H19); PB, peripheral blood (all other analyses for recipients). The grey bar indicates methylation patterns expected for each parental allele based on analysis of methylation in gametes.¹⁰¹