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. 2008 Jan-Mar;4(1):33–41. doi: 10.4161/org.6123

Figure 6.

Figure 6

Neural in vitro differentiation of AG, GG and N ES cells. (A) Phase contrast images of AG, GG and N ES cells at progressive stages of in vitro differentiation. (a–c) embryoid bodies derived from AG, GG and N ES cells (day 4 of differentiation), magnification: 50X. (d–f) pan-neural progenitor cells (day 12 of differentiation), 100X. (B) Immunostaining of pan-neural progenitor cell-derived neuronal and glial cells. Pan-neural progenitor cells were cultured for 13 days under conditions for neural differentiation. (a–c) tubulin-β-III positive neuronal cells (red). (d–f) GFAP+ astroglial cells (green). Nuclei were counterstained with DAPI, magnification: 200X.