Table 1.

New validated RXLR effectors. The new validated effectors are based on two Phytophthora sojae avirulence proteins, PsAvr1a and PsAvr3a, reported by the laboratory of Mark Gijzen, London, Ontario, Canada and their homologs (E value <10⁻⁴)

Description	Accessiona	Species	Evidence	Lengthb	Signal Peptide Lengthb	SignalP v2.0 HMM score	SignalP v2.0 NN score	RXLR Positionc	EER positionc
	ABQ81647,
Avirulence effector protein PsAvr3a	Ps_scaffold_80_R245	P. sojae	Avr effector	111	20	0.998	0.910	43
	ABo47652,
Avirulence effector protein PsAvr1a	Ps_scaffold_1058_F4	P. sojae	Avr effector	121	25	0.999	0.813	54	64
Unknown protein similar to PsAvr1a	Pr_scaffold_103_F268	P. ramorum	homolog	121	21	0.994	0.850	54
Unknown protein similar to PsAvr1a	Pr_scaffold_13_F1570	P. ramorum	homolog	129	25	0.998	0.880	48	68
Unknown protein similar to PsAvr1a	Pr_scaffold_17_F1241	P. ramorum	homolog	112	21	0.997	0.815	51	59
Unknown protein similar to PsAvr1a	Pr_scaffold_207_F26	P. ramorum	homolog	138	23	0.997	0.898	56	69
Unknown protein similar to PsAvr1a	Pr_scaffold_251_R3	P. ramorum	homolog	139	21	1	0.894	54	73
Unknown protein similar to PsAvr1a	Pr_scaffold_26_R566	P. ramorum	homolog	141	21	1	0.898	54	75
Unknown protein similar to PsAvr1a	Pr_scaffold_26_R615	P. ramorum	homolog	152	21	1	0.884	58	86
Unknown protein similar to PsAvr1a	Pr_scaffold_34_F586	P. ramorum	homolog	293	21	1	0.938	52	67
Unknown protein similar to PsAvr1a	Pr_scaffold_50_R933	P. ramorum	homolog	140	23	1	0.822	52
Unknown protein similar to PsAvr1a	Pr_scaffold_52_F517	P. ramorum	homolog	151	22	0.994	0.811	52	70
Unknown protein similar to PsAvr1a	Pr_scaffold_64_F233	P. ramorum	homolog	293	21	1	0.947	52	67
Unknown protein similar to PsAvr1a	Pr_scaffold_64_F343	P. ramorum	homolog	294	21	1	0.932	52	67
Unknown protein similar to PsAvr1a	Pr_scaffold_65_R231	P. ramorum	homolog	162	21	1	0.953	58	83
Unknown protein similar to PsAvr1a	Pr_scaffold_75_F477	P. ramorum	homolog	136	23	0.999	0.870	53	69
Unknown protein similar to PsAvr1a	Pr_scaffold_91_R166	P. ramorum	homolog	154	21	0.999	0.892	57	81
Unknown protein similar to PsAvr1a	Ps_scaffold_118_R508	P. sojae	homolog	98	21	0.998	0.869	54	71
Unknown protein similar to PsAvr1a	Ps_scaffold_122_R489	P. sojae	homolog	125	25	0.999	0.858	48	68
Unknown protein similar to PsAvr1a	Ps_scaffold_27_R1297	P. sojae	homolog	305	21	0.996	0.951	51
Unknown protein similar to PsAvr1a	Ps_scaffold_3_R4103	P. sojae	homolog	130	21	0.994	0.863	54	70
Unknown protein similar to PsAvr1a	Ps_scaffold_36_F644	P. sojae	homolog	137	23	1	0.856	53	74
Unknown protein similar to PsAvr1a	Ps_scaffold_68_F347	P. sojae	homolog	162	21	1	0.898	50	61
Unknown protein similar to PsAvr3a	Pr_scaffold_1497_R5	P. ramorum	homolog	126	19	0.997	0.934	41	56
Unknown protein similar to PsAvr3a	Pr_scaffold_33_F760	P. ramorum	homolog	126	19	0.998	0.942	41	56
Unknown protein similar to PsAvr3a	Pr_scaffold_33_F786	P. ramorum	homolog	125	19	1	0.932	41	56
Unknown protein similar to PsAvr3a	Pr_scaffold_33_R44	P. ramorum	homolog	128	19	0.998	0.942	41	56
Unknown protein similar to PsAvr3a	Pr_scaffold_34_R60	P. ramorum	homolog	127	19	0.997	0.943	41	56
Unknown protein similar to PsAvr3a	Pr_scaffold_6_R2337	P. ramorum	homolog	203	20	1	0.941	43	61
Unknown protein similar to PsAvr3a	Pr_scaffold_6_R2603	P. ramorum	homolog	204	20	1	0.935	43	61
Unknown protein similar to PsAvr3a	Ps_scaffold_106_F265	P. sojae	homolog	131	20	0.999	0.930	45
Unknown protein similar to PsAvr3a	Ps_scaffold_106_R557	P. sojae	homolog	131	20	0.999	0.930	45
Unknown protein similar to PsAvr3a	Ps_scaffold_24_F382	P. sojae	homolog	137	20	1	0.954	44
Unknown protein similar to PsAvr3a	Ps_scaffold_31_F1779	P. sojae	homolog	167	20	1	0.934	43	56
Unknown protein similar to PsAvr3a	Ps_scaffold_31_R1171	P. sojae	homolog	120	20	1	0.950	40	58
Unknown protein similar to PsAvr3a	Ps_scaffold_87_F189	P. sojae	homolog	145	22	1	0.879	43	75
			Means	155.94	21.1	0.99	0.90	48.9	66.6
			Means	158.3	20.7	0.99	0.86	45.0	62.1
			reported by
			Win et al
			(2007)

The two P. sojae avirulence proteins were reported after we applied the gene mining pipeline described in Win et al (2007) and therefore validate the approach. This list of 36 genes complements the 43 validated effectors described in Table 1 of Win et al. (2007).

^aGenBank accession number is provided where available. Otherwise, accession numbers correspond to sequences listed in Table S2 of Win et al (2007).

^bLength in amino acids.

^cPosition counting from N-terminus.