Table 1.
Duration of imbibition (h) | H2O2 content (µmol g DW-1) | CATA1 expression (relative units) | ||
dormant | non-dormant | dormant | non-dormant | |
0 (dry) | 1.37 ± 0.21 | 2.10 ± 0.14 | 100 | 46.0 |
8 | 1.43 ± 0.35 | 4.52 ± 0.65 | 48.0 | 44.6 |
16 | 1.50 ± 0.22 | 8.22 ± 1.26 | 59.3 | 114 |
Hydrogen peroxide content and CATA1 gene expression in axes isolated from dormant and non-dormant sunflower seeds before imbibition (dry) or after 8 and 16 h of imbibition on water at 10°C.. Values are means of five replicates ± SD (H2O2). CATA1 expression was determined by northern blot and is % of the expression determined in dry dormant seeds (means of three measurements).