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. Author manuscript; available in PMC: 2009 Feb 2.
Published in final edited form as: Stem Cell Res. 2007 Oct;1(1):61–74. doi: 10.1016/j.scr.2007.09.004

FIGURE 9.

FIGURE 9

The presence of syncytial-like structures in H9 hESC cultured in the presence of BMP4 for five days. Nuclei are blue. Images are 3D projections of multiple confocal sections. a, H9 cells positive for hCGβ (red) do not have desmosomes (green) separating nuclear areas, suggesting a continuous cytoplasm. b, The network of cytokeratin 8 (red) staining that delineates individual cells outside the hCGβ (green) areas is not apparent within areas staining for hCGβ (green). c, Syncytial-like regions with continuous cytokeratin 8 staining (red) also show intense nuclear staining for the transcription factor GATA2 (green). Scale bars represent 100 μm.