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. Author manuscript; available in PMC: 2009 Feb 2.
Published in final edited form as: J Cell Biochem. 2008 Sep 1;105(1):89–98. doi: 10.1002/jcb.21801

Fig. 4.

Fig. 4

Effects of PGF2α-treatment on the expression of HIF-1α mRNA isoforms in MDI-treated 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were induced to undergo adipocyte differentiation by the standard MDI protocol in the presence of either vehicle or 100 nM PGF2α for the first 48 h of the culture period. Total RNA was isolated at the indicated number of days post-differentiation and the expression of either the HIF-1α I.1 or HIF-1a I.2 mRNA isoforms, or HPRT was analyzed by RT-PCR analysis. Data are representative of three independent experiments.