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. Author manuscript; available in PMC: 2009 Feb 2.
Published in final edited form as: J Cell Biochem. 2008 Sep 1;105(1):89–98. doi: 10.1002/jcb.21801

Fig. 5.

Fig. 5

Effects of a constitutively active HIF-1α mutant on 3T3-L1 preadipocyte differentiation. A: 3T3-L1 preadipocytes transduced with either a GFP control retrovirus, or a retrovirus encoding caHIF-1α were induced to undergo adipocyte differentiation by the standard MDI protocol and stained with Oil Red O to determine the extent of differentiation at day 10. B: Whole cell extracts prepared from either GFP control- or caHIF-1α-virus transduced 3T3-L1 preadipocytes at either day 0 or day 10 post-MDI treatment were analyzed by immunoblotting for expression of PPARγ and C/EBPα. The expression of actin was determined as a control. Data are representative of at least two independent experiments.