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. 2008 Jun;3(6):401–403. doi: 10.4161/psb.3.6.5429

Figure 1.

Figure 1

Non-selective outward current in barley root epidermal protoplasts. (A) Typical current traces of NORC-like channels in the whole cell configuration (cv. Franklin, capacitance, 9 pF). The voltage pulse protocol is shown at the top. (B) A current-voltage relation derived from the experiment in (A). Inset shows I/V curve for the tail currents (NORC was pre-activated by the voltage pulse to +80 mV, followed by a pulses to test potentials between −100 and +20 mV). Equilibrium potentials for Cl− (ECl) and K+ (EK) are indicated. (C) Comparison of average NORC-like current per protoplast at +60 mV. Data are means +/− SE; number of successful recordings (protoplasts expressing NORC) are indicated for each cultivar. Total numbers of protoplasts tested were 35 for Gairdner, 19 for Franklin (two salt-sensitive cultivars), 30 for ZUG 293 and 23 for CM72 (two salt-tolerant cultivars).