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. 2008 Jun;3(6):420–422. doi: 10.4161/psb.3.6.5471

Table 1.

Relative levels of GL1 and HSP70 expression4

Gene Tissue Relative Quantity1 Shoot over Leaf
GL1 Col shoot2 1.000 ± 0.063 490
GL1 Col leaf3 2.04 × 10−3 ± 0.045 × 10−3
GL1 sstsim shoot 1.586 ± 0.093 1.5
GL1 sstsim leaf 1.043 ± 0.109
GL1 sst cycD3;1 shoot 2.666 ± 0.701 19.6
GL1 sst cycD3;1 leaf 0.136 ± 0.011
HSP705 Col shoot2 1.000 ± 0.018 1.09
HSP70 Col leaf 0.915 ± 0.026
HSP70 sstsim shoot 1.195 ± 0.099 1.2
HSP70 sstsim leaf 1.028 ± 0.051
HSP70 sst cycD3;1 shoot 1.578 ± 0.096 1.85
HSP70 sst cycD3;1 leaf 0.852 ± 0.082
1

All quantities were normalized to the level of expression obtained for Col shoot. GL1 and HSP70 values were normalized separately. Mean ± standard deviation values are shown derived from 2 replicates of each tissue.

2

All young shoot tissue contained just the apex and emerging leaves that lacked petioles.

3

Third leaves were dissected from seedlings just at the stage when the leaves were beginning to develop petioles.

4

All reaction conditions and primers are described in Marks et al., 2007.

5

The HSP70 gene was used an internal control of mRNA integrity, as previous analyses have shown that the expression of this gene varies little from tissue to tissue under normal growth conditions.