Table 1.
Gene | Tissue | Relative Quantity1 | Shoot over Leaf |
GL1 | Col shoot2 | 1.000 ± 0.063 | 490 |
GL1 | Col leaf3 | 2.04 × 10−3 ± 0.045 × 10−3 | |
GL1 | sstsim shoot | 1.586 ± 0.093 | 1.5 |
GL1 | sstsim leaf | 1.043 ± 0.109 | |
GL1 | sst cycD3;1 shoot | 2.666 ± 0.701 | 19.6 |
GL1 | sst cycD3;1 leaf | 0.136 ± 0.011 | |
HSP705 | Col shoot2 | 1.000 ± 0.018 | 1.09 |
HSP70 | Col leaf | 0.915 ± 0.026 | |
HSP70 | sstsim shoot | 1.195 ± 0.099 | 1.2 |
HSP70 | sstsim leaf | 1.028 ± 0.051 | |
HSP70 | sst cycD3;1 shoot | 1.578 ± 0.096 | 1.85 |
HSP70 | sst cycD3;1 leaf | 0.852 ± 0.082 |
All quantities were normalized to the level of expression obtained for Col shoot. GL1 and HSP70 values were normalized separately. Mean ± standard deviation values are shown derived from 2 replicates of each tissue.
All young shoot tissue contained just the apex and emerging leaves that lacked petioles.
Third leaves were dissected from seedlings just at the stage when the leaves were beginning to develop petioles.
All reaction conditions and primers are described in Marks et al., 2007.
The HSP70 gene was used an internal control of mRNA integrity, as previous analyses have shown that the expression of this gene varies little from tissue to tissue under normal growth conditions.