Figure 4.

The orientation of putative loop regions (L2, L3, L4, L5) of ALMT1 expressed in 293T cells. Cells transiently expressing the ALMT1 construct tagged with His at L2 (L2h, shown with the amino acid positions of the replacement to His tag), L3 (L3h), L4 (L4h) and L5 (L5h) were identified with the AF594 fluorescence obtained with the antipeptide antibody against CTHp, in the presence or absence of Triton X-100. These cells were simultaneously tested for the accessibility of the anti-His antibody (detected by AF488 fluorescence). Left panels, AF488 fluorescence; Center panels, AF594 fluorescence; Right panels, the phase contrast image overlaid with AF488 fluorescence. Bar = 20 µm.