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. 2007 Nov-Dec;2(6):467–472. doi: 10.4161/psb.2.6.4801

Table 1.

His-tagged ALMT1 proteins created by replacement of several amino acids with His residues

Name of Tag Site Tag Site1 Amino Acid Sequences at Tag Site1
Amino Acid Position Original Sequences Mutant Sequences2
L2h Loop 2 76–79 FNGL H*H76HHH79H*
L3h Loop 3 103–108 SKGLNR H103HHHHH108
L4h Loop 4 130–135 ERCGDQ H130HHHHH135
L5h Loop 5 159–164 PEIKAK H159HHHHH164
1

The sites of the loops and the His tags in ALMT1 protein are shown in Figures 1 and 4, respectively.

2

In L2h, two additional histidine residues (shown with *) were inserted.