Figure 1.

H₂O₂ accumulation in elicited Rubia tinctorum suspension culture cells. (A) Timing of H₂O₂ formation in untreated and elicited cultures. Mock (Mock), Botrytis cinerea fungal cell wall hydrolysate (Bot), jasmonic acid (JA), salicylic acid (SA). The 0.1 value of the log scale indicate 0 minuntes, other time-points are 2, 4, 6, 8, 10, 20, 30, 40, 50, 60, 120, 240, 480, 720, 1140, 2160, 2880 minutes. (B) Ce perhydroxids detected after 30 min fungal elicitor treatment. (C) Ce precipitates in the cell wall after 8 hours fungal elicitor treatment. v, vacuole; t, tonoplast; cw, cell wall; m, mitochondrion; Bar: 200 nm.