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. 2008 Jan-Mar;2(1):37–41. doi: 10.4161/pri.2.1.6436

Figure 3.

Figure 3

SDS-PAGE profiles of the eight spontaneous mutants obtained. Cell extract from each of the eight mutants of interest (M-1 through M-8) was prepared and subjected to SDS-PAGE as described in Materials and Methods. C and G represent control BL21/pGEX-SUP35NM (producing GST-Sup35NM) and BL21/pGEX-4T-3 (producing GST), respectively. SM1 and SM2 are two different mixtures of size markers. A band corresponding to a position of 55 kDa is marked with open or solid arrow head; for explanation, see text.