Skip to main content
. 2007 Apr-Jun;1(2):144–153. doi: 10.4161/pri.1.2.4630

Figure 6.

Figure 6

A nucleus-targeted GFPN104 results in decreased cell viability. Wild-type, hsp104Δ and rnq1Δ cells carrying the control vector or plasmids encoding galactose-inducible, NLS or NES-tagged GFPC and GFPN104 were grown to mid-log phase under repressed conditions. Cultures were diluted and ∼500 cells were plated on appropriate selective media containing either glucose (repressed) or galactose (induced) as the carbon source. The numbers of colonies were counted after 48-hour (glucose) or 96-hour (galactose) incubation at 30°C. Viability of cells bearing each plasmid was calculated as the quotient of the number of colonies on the galactose plate divided by the number of colonies on the glucose plate. For each strain, the viabilities of cells expressing GFPC and GFPN104 were normalized against the viability of cells harboring the vector alone, yielding the relative viability. (A) Comparison of viability between wild-type cells containing the vector control, the NLS-tagged GFPC and GFPN104, and the NES-tagged GFPC and GFPN104. (B) Comparison of the effect of NLS-GFPC and NLS-GFPN104 on cell viability relative to the vector control in wild-type, hsp104Δ and rnq1Δ strains. **p value ≤ 0.005, ***p value ≤ 0.001.