Figure 2.

SA-induced disease resistance in Col-0 and gh3.5-1D. (A) Disease symptom of Pst DC3000 in mock and SA-treated Col-0 (left) and gh3.5-1D (+/−) (right). Five-week-old plants of the wildtype and mutants were sprayed with 1 mM SA and further inoculated with Pst DC3000 at a dose of 10⁵ cfu ml⁻¹ (OD600 = 0.0002). All controls were infiltrated with 10 mM MgCl₂ for mock inoculation. Photos were taken at 3 dpi. Similar results were observed in two independent experiments. (B) Growth of Pst DC3000 in SA-treated leaves of Col-0 and gh3.5-1D. Bacterial growth assay was performed at 0 and 3 days after inoculation in Col-0 and gh3.5-1D (+/−). All values are means ± SE (n = 6). The SA-induced resistance was significantly stronger (p < 0.05) in gh3.5-1D than the wldtype. cfu, colony-forming units. Similar results were observed in two independent experiments.