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Comparison of binding of apt #1 and bPrP using competitive binding assay. The #1 mutants (m7, m8) contained substitutions (shown underlined) in the conserved region (GGA)4 as follows: m7: 5′-GGUGGAGGAGGA-3′; m8: 5′-GGUGGUGGUGGU-3′. The competitor RNAs were added in various concentrations with 0, 1, 5 and 10-fold greater molar concentrations of labeled apt #1. Binding assay was performed in the presence of 10 mM KCl (A) and in the absence of KCl (B).
