Table 1.

In vitro selection conditions for recombinant bPrP

Round of selection	RNA (µM)	bPrP (µM)	tRNA (µM)	Anti-PrP RNA aptamer16 (µM)	Time (min)
1	10	2	0	0	60
2	6	0.4	10	0	30
3	4	0.2	40	8	20
4	3	0.1	100	16	15
5	2	0.3	100	32	10
6	2	0.05	100	48	10
7	2	0.05	*6	0	10
8**	2	0.05	100	64	10
9**	2	0.05	100	64	10
10	6	0.05	100	96	10

^*U₁₃ was used instead of tRNA.

^**The mutagenic PCR was introduced.