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Detection of PK-digested β-PrP by Western blotting. Images of chemiluminescence detection using clone 4–9 and the anti-PrP antibody 7D9 are shown. β-PrP at a concentration of 0.2 mg/mL was treated with PK at various concentrations. The numbers show the concentration of PK: (1) no PK, (2) 4 ng/mL, (3) 8 ng/mL, (4) 40 ng/mL, (5) 80 ng/mL.
