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Only long-term ethylene treatment triggers PA accumulation in Arabidopsis suspension cells. Arabidopsis seedlings (A and C) or suspension cells (B) were labeled with 32Pi and subsequently treated with 500 µM ethephon (A and B) or 100 µM ACC (C). As a positive control for PA accumulation, seedlings were treated with 500 mM NaCl (C). Phospholipids were extracted, separated by alkaline TLC, visualized by autoradiography and quantified by PhosphoImaging.
