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. 2009 Feb;8(2):215–225. doi: 10.1074/mcp.M800187-MCP200

Fig. 1.

Fig. 1.

Synopsis of lysine acetylation proteomics. A, schematic representation of the sequential steps used for global profiling of lysine acetylation in E. coli. B, Western blotting analysis of protein lysate from E. coli with anti-acetyllysine antibody from ImmuneChem Inc. Lane 1, 30 μg of whole cell lysate from E. coli probed with anti-acetyllysine antibody; lane 2, competition with acetylated BSA (300 μg/ml). C, an example of MS and MS/MS analysis of a lysine-acetylated peptide for peptide identification and mapping of acetyllysine site in E. coli. Left panel, full MS spectrum at a retention time of 63.69 min. Right panel, MS/MS spectrum of m/z 1261.2, which identifies acetylated peptide YYQGTPSPVK*HPELTDMVIFR in isocitrate dehydrogenase, a metabolic protein in E. coli.