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. 2009 Feb;8(2):302–315. doi: 10.1074/mcp.M800058-MCP200

Fig. 5.

Fig. 5.

Validation of associating partners of cardiac and hepatic 20 S proteasomes. A, cardiac and hepatic 20 S proteasome was run on a native gel, transferred to nitrocellulose, and probed with polyclonal anti-NEDD8 and monoclonal anti-β7. B, identification of a peptide from NEDD8 present in the BN-PAGE 20 S band using LC-MS/MS analysis. C, immunoblot of native gel to verify interaction of PP1 with the heart 20 S proteasomes. D, identification of a peptide from PP1 present in the BN-PAGE 20 S band using LC-MS/MS analysis.