Figure 4.

Surface Area Algorithms for Image Analysis. (A) Custom-written, MATLAB algorithms were developed to measure surface area of cells (in pixels). Blue text indicates an integrated MATLAB function and black is used for all other text and commands. (B) Sample phase contrast images (top row) and corresponding micrographs of thresholded surface area images (middle row) are presented for a small, medium, and large cell. The calculated number of pixels that corresponds to each cell is also listed for reference (bottom row).