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. 2008 Jul-Sep;4(3):137–143. doi: 10.4161/org.4.3.6498

Figure 3.

Figure 3

(A–C) Phase contrast photomicrographs of either (A) isolated whole mesonephros, including the Wolffian duct, (B) isolated WD in which the mesonephric tubules, along with most of the non-epithelial mesoderm is removed or (C) isolated WD cleared of all surrounding mesoderm before in vitro culture (“naked” epithelial tube) suspended within a 3D extracellular matrix gel. The cultures were grown for 3 days in either (A and B) DME/F12 supplemented with FBS and soluble grow factors [(A) 10 ng/ml GDNF; (B) 125 ng/ml GDNF and 250 ng/ml FGF1] or (C) BSN conditioned medium supplemented with 125 ng/ml GDNF and 250 ng/ml FGF1 ; (Scale bar: 500 µm.) (From ref. 20).