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. 2008 Jul-Sep;4(3):137–143. doi: 10.4161/org.4.3.6498

Figure 6.

Figure 6

(A and B) Phase contrast photomicrographs demonstrating the ability to microdissect and propogate cultured isolated rat embryonic UBs. (A) UB cultured for 7 days in BSN conditioned medium (supplemented with 10% FBS, 125 ng/ml GDNF and 250 ng/ml FGF1) were dissected and subdivided into thirds and resuspended within 3D ECM gels. This subdivided UBs were cultured for an additional 8 days using the same culture conditions. (B) One of these second generation cultured was further subdivided, resuspended and cultured for an additional 8 days in BSN conditioned media (supplemented with 10% FBS, 125 ng/ml GDNF and 250 ng/ml FGF1). (C) A schematic representation of the procedure for isolated UB propagation indicates the potential for generating a large number of UBs from a single progenitor UB. (From ref. 30).