Figure 5.

Propagons in pairs of mother and daughter cells taken from a culture of ψ⁺ cells in which Hsp104 had been overexpressed for 20 hours. The mothers and daughters were separated after cytokinesis and placed on 3 mM guanidine hydrochloride as described in reference ⁶. The figure illustrates malsegregation in a minority of the dividing cells, never observed in normal cultures. Thirty-six percent of the colonies from this culture were ψ^- or sectored.