Figure 3.
PrPSc accumulation in the brain of treated animals. (A) PrPSc was enriched from brain homogenates by precipitation with g5p and digested or not with PK (-/+ lanes). PrPSc was evaluated at the end of the treatement (lanes 1–10) or at the terminal stage of disease (lanes 11 and 12). (B) PrPSc was purified from brains of animals at the end of treatments (left set of bars), or at terminal stage (right set of bars) by the purification technique described in the methods, and then detected by sandwich ELISA.