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. Author manuscript; available in PMC: 2009 Feb 2.
Published in final edited form as: J Comp Neurol. 2006 May 1;496(1):13–26. doi: 10.1002/cne.20885

Figure 2.

Figure 2

Electron micrographs of the localisation of BED (biotin ethylenediamine) in blood vessels deep inside the cortex of a 2 month old opossum 10min after an intravenous injection. Similar staining is found after an intravenous injection of biotin-dextran (BDA3000).

A) Low power micrograph showing two paired vessels with abundant reaction product within the lumen. No reaction product is visible in the surrounding tissue. Pairs of arteries and veins are characteristic of the vascular pattern in marsupial brains (Wislocki and Campbell, 1937).

B) High power micrograph of an interendothelial cleft showing that the tight junctions in the young adult restrict the passage of BED through the cleft (arrowhead). Scale bars are 4µm in A, 300nm in B.