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. 1984 Jan;43(1):442–444. doi: 10.1128/iai.43.1.442-444.1984

Malaria vaccine antigen(s): detergent solubilization, partial isolation, and recovery of immunoprotective activity.

L E D'Antonio, H Keshavarz-Valian, N E Alger
PMCID: PMC263461  PMID: 6360908

Abstract

Plasmodium berghei-infected erythrocytes were solubilized with the nonionic detergent n-octyl glucoside and membrane-containing P. berghei fragments with either n-octyl glucoside or the ionic detergent sodium deoxycholate. Unsolubilized material was separated by centrifugation, and the detergent was removed from the respective supernatants by gel filtration. Reaggregated components in the respective void volume eluates acted as a specific vaccine in recipient mice, resulting in a dose-related accelerated elimination of infection and increased survival after challenge with the homologous parasite.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alger N. E., Branton M., Harant J., Silverman P. H. Plasmodium berghei NK65 in the inbred A-J mouse: variations in virulence of P. berghei demes. J Protozool. 1971 Nov;18(4):598–601. doi: 10.1111/j.1550-7408.1971.tb03382.x. [DOI] [PubMed] [Google Scholar]
  2. Baron C., Thompson T. E. Solubilization of bacterial membrane proteins using alkyl glucosides and dioctanoyl phosphatidylcholine. Biochim Biophys Acta. 1975 Mar 25;382(3):276–285. doi: 10.1016/0005-2736(75)90270-9. [DOI] [PubMed] [Google Scholar]
  3. Beckwith R., Schenkel R. H., Silverman P. H. Qualitative analysis of phospholipids isolated from nonviable Plasmodium antigen. Exp Parasitol. 1975 Apr;37(2):164–172. doi: 10.1016/0014-4894(75)90067-3. [DOI] [PubMed] [Google Scholar]
  4. Clark I. A., Allison A. C., Cox F. E. Protection of mice against Babesia and Plasmodium with BCG. Nature. 1976 Jan 29;259(5541):309–311. doi: 10.1038/259309a0. [DOI] [PubMed] [Google Scholar]
  5. Clark I. A., Cox F. E., Allison A. C. Protection of mice against Babesia spp. and Plasmodium spp. with killed Corynebacterium parvum. Parasitology. 1977 Feb;74(1):9–18. doi: 10.1017/s003118200004748x. [DOI] [PubMed] [Google Scholar]
  6. D'Antonio L. E. Plasmodium berghei: vaccination of mice against malaria with heat inactivated parasitized blood. Exp Parasitol. 1972 Feb;31(1):82–87. doi: 10.1016/0014-4894(72)90050-1. [DOI] [PubMed] [Google Scholar]
  7. D'Antonio L. E. Plasmodium: a resumé of the isolation of the vaccine fraction by the French pressure cell technique. Exp Parasitol. 1972 Feb;31(1):75–81. doi: 10.1016/0014-4894(72)90049-5. [DOI] [PubMed] [Google Scholar]
  8. D'Antonio L. E., Spira D. T., Fu R. C., Dagnillo D. M., Silverman P. H. Malaria resistance: artificial induction with a partially purified plasmodial fraction. Science. 1970 May 29;168(3935):1117–1118. doi: 10.1126/science.168.3935.1117. [DOI] [PubMed] [Google Scholar]
  9. D'Antonio L. E., Spira D. T., Silverman P. H. Model system for study of artificially induced resistance to malaria. Nature. 1969 Aug 2;223(5205):507–509. doi: 10.1038/223507a0. [DOI] [PubMed] [Google Scholar]
  10. D'Antonio L. E. Vaccination against malaria: evaluation of a plasmodial vaccine fraction and a preliminary study of some immunologic adjuvants in a primate model system: a brief report. J Am Osteopath Assoc. 1974 Apr;73(8):649–652. [PubMed] [Google Scholar]
  11. D'Antonio L. E., Von Doenhoff A. E., Jr, Fife E. H., Jr A new method for isolation and fractionation of complement fixing antigens from Plasmodium knowlesi. Proc Soc Exp Biol Med. 1966 Oct;123(1):30–34. doi: 10.3181/00379727-123-31394. [DOI] [PubMed] [Google Scholar]
  12. De Grip W. J. Thermal stability of rhodopsin and opsin in some novel detergents. Methods Enzymol. 1982;81:256–265. doi: 10.1016/s0076-6879(82)81040-9. [DOI] [PubMed] [Google Scholar]
  13. Grothaus G. D., Kreier J. P. Isolation of a soluble component of Plasmodium berghei which induces immunity in rats. Infect Immun. 1980 Apr;28(1):245–253. doi: 10.1128/iai.28.1.245-253.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holder A. A., Freeman R. R. Immunization against blood-stage rodent malaria using purified parasite antigens. Nature. 1981 Nov 26;294(5839):361–364. doi: 10.1038/294361a0. [DOI] [PubMed] [Google Scholar]
  15. Khansari N., Segre M., Segre D. Immunosuppression in murine malaria: a soluble immunosuppressive factor derived from Plasmodium berghei-infected blood. J Immunol. 1981 Nov;127(5):1889–1893. [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Playfair J. H., De Souza J. B., Cottrell B. J. Protection of mice against malaria by a killed vaccine: differences in effectiveness against P. yoelii and P. berghei. Immunology. 1977 Oct;33(4):507–515. [PMC free article] [PubMed] [Google Scholar]
  18. ROBINSON D. R., JENCKS W. P. THE EFFECT OF CONCENTRATED SALT SOLUTIONS ON THE ACTIVITY COEFFICIENT OF ACETYLTETRAGLYCINE ETHYL ESTER. J Am Chem Soc. 1965 Jun 5;87:2470–2479. doi: 10.1021/ja01089a029. [DOI] [PubMed] [Google Scholar]
  19. Richards W. H., Williams S. G. The removal of leucocytes from malaria infected blood. Ann Trop Med Parasitol. 1973 Jun;67(2):249–250. doi: 10.1080/00034983.1973.11686885. [DOI] [PubMed] [Google Scholar]
  20. Speer C. A., Silverman P. H., Barr M. L. Ultrastructural study of Plasmodium knowlesi antigen used in vaccination of rhesus monkeys. J Protozool. 1976 Aug;23(3):437–442. doi: 10.1111/j.1550-7408.1976.tb03806.x. [DOI] [PubMed] [Google Scholar]
  21. Yu B. P., DeMartinis F. D., Masoro E. J. Isolation of Ca++-sequestering sarcotubular membranes from rat skeletal muscle. Anal Biochem. 1968 Sep;24(3):523–530. doi: 10.1016/0003-2697(68)90160-7. [DOI] [PubMed] [Google Scholar]

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